Untertitel:
Methods and Applications
Erscheinungsdatum:
25.09.2002
Introducing the concept of artificial DNA, this book presents state-of-the-art research on the process, trends, and application of artificial DNA. The text covers methods of oligonucleotide synthesis and direct uses of synthetic DNA, as well as methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. It explains how artificial DNA may be used to develop products that will achieve an intended purpose.Covering applications in synthetic DNA in various fields and industries, the book offers a comprehensive review that helps readers understand the strategy, design, and applications of synthetic oligonucleotides.
Autorentext
Yury E. Khudyakov, Howard A. Fields
Zusammenfassung
Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening fo
Inhalt
Chemical Synthesis of Oligonucleotides: From Dreams to Automation. Artificial Hybridization Probes. Peptide Nucleic Acid (PNA): A DNA Mimic with a Pseudopeptide Backbone. Synthetic DNA in Amplification Reactions. Artificial Nucleic Acids and Genomic Profiling. Site-Directed Mutagenesis. Mimotopes. Artificial Genes for Chimeric Virus-like Particles. DNA Vaccines. Recombinant and Artificial Antigens as Diagnostic Reagents. Recombinant Antibodies.
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